Evaluation of Vitek and Disk Diffusion Susceptibility Testing of Enterobacteriaceae against Piperacillin-Tazobactam and Methicillin-Resistant Staphylococcus aureus against Trimethoprim-Sulfamethoxazole / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 160-164, 2005.
Article
in Korean
| WPRIM
| ID: wpr-68469
ABSTRACT
BACKGROUND:
The aim of this study was to evaluate the Vitek system and the disk diffusion method for susceptibility testing of Enterobacteriaceaeagainst piperacillin-tazobactam (TZP) and methicillin-resistant Staphylococcus aureus (MRSA) against trimethoprim-sulfamethoxazole (SXT)using the broth microdilution method as the reference.METHODS:
Using the Vitek system and the disk diffusion method, we tested 96 isolates of Enterobacteriaceae (48 Escherichia coli, 26 Klebsiella pneumoniae, 8 Serratia marcescens, 6 Enterobacter cloacae, 2 E. aerogenes, 2 K. oxytoca, 2 Citrobacter freundii, 2 Pantoea agglomerans) and 61 isolates of MRSA for susceptibity against TZP and SXT, respectively; the broth microdilution of National Committee for Clinical Laboratory Standards was used as the reference method.RESULTS:
In the susceptibility testing of Enterobacteriaceae against TZP, Vitek system yielded 10 (10%) minor errors, and the disk diffusion method one (1%) very major and 13 (14%) minor errors. For the MRSA against SXT, the rate of categorical agreement between the reference method and the Vitek or the disk diffusion method was both 100%. The rates of agreement between the reference method and the Vitek system in term of MICs (within +/-1 dilution) were 93% and 98% in the susceptibility testing of Enterobacteriaceae against TZP and MRSA against SXT, respectively.CONCLUSION:
Both Vitek system and disk diffusion method showed an acceptable level of accuracy for the susceptibility test of Enterobacteriaceaeagainst TZP and MRSA against SXT.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Serratia marcescens
/
Trimethoprim, Sulfamethoxazole Drug Combination
/
Methicillin Resistance
/
Enterobacter cloacae
/
Citrobacter freundii
/
Pantoea
/
Diffusion
/
Enterobacteriaceae
/
Escherichia coli
/
Methicillin-Resistant Staphylococcus aureus
Type of study:
Practice guideline
Language:
Korean
Journal:
Korean Journal of Clinical Microbiology
Year:
2005
Type:
Article
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