The expression of UCP2 in myocardium of rats with intestinal ischemic preconditioning / 医学研究生学报

ABSTRACT

Objective:To discuss the protective effect of intestinal ischemic preconditioning(iIPC) on the myocardium and the role of uncoupling protein 2(UCP2) in myocardium with iIPC. Methods: The animal model was built by superior mesenteric artery ligation of rats.Then they were subjected to a sustained coronary occlusion of 30 min and reperfusion of 180 min.We evaluated the degree of the myocardial damage and used RT-PCR method to check the relative content of UCP2 mRNA.The IR control group underwent the coronary occlusion/ reperfusion with no intestinal ischemia and the blank group was sham-operated. Results:①Compared to the blank group,the IR control group showed much heavier damage in the ultramicro structure.And for the iIPC group, the damage in the ultramicro structure 0 h and 24 h after iIPC was lighter than that of the IR control group.②Compared to the expression of the UCP2 mRNA in the myocardium of rats in the IR control group,the expressions of iIPC 0 h,6 h,12 h,24 h,48 h groups increased substantially(P