Enhancement of Lycopene Production by Blakeslea trispora Using Oxygen-vectors and Surface Active Agents / 微生物学通报

ABSTRACT

Addition of oxygen-vectors(n-dodecane,n-hexane,hydrogen peroxide) to fermentation medium was recognized as a method of enhancing oxygen transfer and promoting lycopene yield by Blakeslea trispora fermentation.Higher lycopene production was observed in shake flask containing 1%(v/v) n-hexane,n-dodecane and 150 L/100mL hydrogen peroxide as compared to shake flasks without oxygen-vectors.The result of assays indicated that when oxygen-vectors (n-dodecane,n-hexane,1%,v/v) and hydrogen peroxide(50 L/100mL) were added to the 0-day and 1-day old culture of Blakeslea trispora the production of lycopene were 25.32%,72.84% and 40.35% higher than those without oxygen-vectors addition respectively.The production of lycopene increased 114.83% when n-dodecane and surface active agents were used at the same time.