Cloning and Expression of Gene Encoding (R)-specific Carbonyl Reductase from Candida parapsilosis CCTCC M203011 in Escherichia coli / 微生物学通报

ABSTRACT

The gene which encodes (R)-specific carbonyl reductase(rCR) from Candida parapsilosis CCTCC M203011 was cloned.The cloned sequence includes an open reading frame (ORF) consisting of 1011bp, encoding a protein of 336 amino acids, with a molecular weight of 35.9 kD.The nucleotide sequence showed 99% similarity to those of the other members of the alcohol dehydrogenase superfamily.A recombinant Escherichia coli JM109 strain harboring the expression plasmid, produced (R)-1-phenyl-1,2-ethanediol(100%e.e,80.4%yield) from ?-hydroxyacetophenone without any additive to regenerate NAD~ + from NADH.