Antiangiogenesis Gene Therapy Using Adenovirus-mediated Antisense-VEGF in Glioblastoma Multiforme / 대한암학회지
Journal of the Korean Cancer Association
;
: 764-774, 2000.
Article
in Korean
| WPRIM
| ID: wpr-68511
ABSTRACT
PURPOSE:
Vascular endothelial growth factor (VEGF) is a major positive effector of angiogenesis. We investigated the mechanism of tumor growth inhibition by adenoviral transfer of antisense- VEGF in glioma and the role of VEGF for in vivo growth of human glioma cells according to the stage of the tumor growth. MATERIALS ANDMETHODS:
Replication-deficient adenoviral vector containing the VEGF cDNA in an antisense orientation (Ad5CMV-alphaVEGF) were constructed to increase the in vivo applicability of antisense sequence. The effect of Ad5CMV-alphaVEGF was studied in vitro and in vivo with human glioma cell line U-87 MG. Immunohistochemical staining of the subcutaneous tumor with anti-VEGF antibody and CD34 antibody were performed to compare VEGF protein expression and the microvessel count respectively.RESULTS:
The growth curve of U-87 MG cells treated with Ad5CMV-alphaVEGF remained as same as that of mock-infected and Ad5(dl312)-infected U-87 MG cells in vitro, suggesting that Ad5CMV-alphaVEGF does not have direct cytotoxic effect. The growth of subcutaneous human glioma xenografts was inhibited by early intratumoral injection of Ad5CMV-alphaVEGF. Immuno histochemical staining of tumors showed that VEGF protein expression and mean microvessel counts were decreased in early Ad5CMV-alphaVEGF treatment group.CONCLUSION:
The efficient down-regulation of VEGF produced by tumor cells using Ad5CMV- alphaVEGF in early stage of glioma growth has an antitumor effect in vivo through antiangiogenic mechanism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genetic Therapy
/
Down-Regulation
/
Cell Line
/
Adenoviridae
/
DNA, Complementary
/
Glioblastoma
/
Vascular Endothelial Growth Factor A
/
Microvessels
/
Heterografts
/
Glioma
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Cancer Association
Year:
2000
Type:
Article
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