Evaluation and comparison of MRSA 1D chromogenic agar medium and other 3 methods for detecting and identifying methicillin-resistant Staphylococcus aureus / 中华检验医学杂志

ABSTRACT

Objective To evaluate MRSA ID chromogenic agar medium for detecting and identifying methieillin-resistant Staphylococcus aureus(MRSA).Methods 118 Staphylococcus spp. clinical isolates(including 108 Staphylococcus and 10 coagulase-negative Staphylococcus(CNS)were identified by Bacteria Auto-Identification System.MRSA ID Chromogenic Agar,oxacillin screening agar, and cefoxitin disk diffusion method were compared for detecting methicillin-resistant Staphylococcus aureus (MRSA).Meanwhile,detection of mecA gene by polymerase chain reaction(PCR)was considered as the gold standard.Escheriehia coli ATCC25922,Staphylococcus aureus ATCC25923,Pseudomonas aeruginosa ATCC27853,Enterococcus faecalis ATCC29212 were used for interference test.Results meeA gene of 108 Staphylococcus was detected by PCR and 80 strains were positive.Compared with PCR,the agreement of screening MRSA by MRSA ID chromogenic agar,oxacillin screening agar and cefoxitin disk diffusion method were 100%,97.5%,and 100%,respectively.Only one methicillin-sensitive Staphylococcus(MSSA)and one CNS were shown in wrong color and became reseda micro-colonies on the MRSA ID chromogenic agar. Standard references strains did not grow on the MRSA ID chromogenie agar.Conclusion MRSA ID chromogenic agar can be used to rapidly and initiatively detect MRSA,which is useful to take quick infection control measures.