Technologies for Surface Display of Lipase / 中国生物工程杂志

ABSTRACT

Lipase is a kind of widely used hydrolase.Surface display is an efficient method of highthroughput screening for protein engineering of lipase.Besides,lipase displaying on surface of microorganism has many advantages,such as higher stability against high temperature and organic solvent,compared with free lipase,so the host strain displaying lipase can be used as wholecell biocatalyst,which has some advantages compared with traditional immobilization of lipase.There are three kinds of host strain for displaying lipasephage,bacteria and yeast.The surface display of lipase in the three display systems were systematically discribed,and their current uses and possible trends in the future were discussed also.