Isolation and Characterization of a ?-tubulin Gene from Trichoderma Harzianum / 微生物学通报
Microbiology
; (12)1992.
Article
in Zh
| WPRIM
| ID: wpr-685694
Responsible library:
WPRO
ABSTRACT
Based on a ?-tubulin gene EST sequence from Trichoderma harzianum mycelium cDNA library,a 1.74kb ?-tubulin gene coding region,together with a 1.5kb 5'-flanking region and a 1.0kb 3'-flanking region was cloned from T.harzianum by IPCR methods.The gene encodes a 446 amino acid polypeptide,which shows a high degree of homology with other fungal ?-tubulins.A three-dimensional model of the T.harzianum ?-tubulin was built based on the crystal structure of bovine tubulin using homology modeling method.Tertiary protein structure of the ?-tubulin should provide a basis for understanding a significant body of research on tubulin's properties and interactions with antimicrotubule agents.
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Index:
WPRIM
Language:
Zh
Journal:
Microbiology
Year:
1992
Type:
Article