Effect of bilirubin on the anti-oxidative stress ability of red cell membrane in rats undergoing high intensity training / 中国组织工程研究

ABSTRACT

AIM:To observe the effect of supplementing bilirubin on the anti-oxidative stress ability of red cell membrane in training rats through establishing animal models of high-intensity training. METHODS:The experiment was performed in the physiological laboratory of Physical Institute of Jiangxi Normal University from December 2004 to January 2005.①After one week of adaptive feeding,24 male SD rats were randomly divided into quiet control group,high intensity sports training and physiological saline group(training group),high intensity sports training and bilirubin group(training and supplementing group)with 8 animals in each group.②The training group and the training and supplementing group did the running on the platform with the slope of 0?at the first three weeks and 5?at the fourth week,6 days every week expect Sunday.The training and supplementing group was intragastrically infused with bilirubin by 40mg/kg body mass at 17:00-18:00 after training every day,and the other groups were given physiological saline,six times every week.Anticoagulatory blood about 2mL of heparin was harvested to prepare red cell membrane sample and measure the relevant indexes on the 4~(th)Sunday.③The serum total bilirubin level was determined using automatic biochemical analysis instrument;the malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)of red cell membrane by TBA;the activity of superoxide dismutase(SOD)of red cell membrane using the method of Xanthine oxidase.All the treatment for animals was based on the ethical standards. RESULTS:All 24 rats were involved in the result analysis.①The serum total bilirubin level in training group was lower than quiet control group(P0.05).②The MDA of the red cell membrane in the training group was higher than the control and training and supplementing group(P0.05).③The activity of SOD in training group was lower than quiet control group(P0.05),but the training and supplementing group was higher than the control group and the training group(P