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Study on Heterologous Expression of Penicillinase Gene and the Penicillinase Degrading Residual Penicillin in Milk / 微生物学通报
Microbiology ; (12)1992.
Article in Zh | WPRIM | ID: wpr-685898
Responsible library: WPRO
ABSTRACT
To obtain a number of penicillinases and degrade penicillin in milk by using the penicillinases,the gene encoding penicillinase was amplified by PCR from Bacillus cereus ATCC10987,cloned into pET28a(+) ,transformed into E. coli BL21;analysis of SDS-PAGE and penicillinase activity of the recombinant protein were done under induction of IPTG and the result showed that the maximum penicillinase activity reached 480 U/mL;the purity of penicillinase purified by Ni2+ Purification System was more than 90%;the immobilized penicillinases were obtained by sodium periodate method and the residual quantity of penicillin in milk(containing 0.5 U penicillin G/mL) was less than 4 ppb after degraded by the immobilized penicillinase.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Microbiology Year: 1992 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Microbiology Year: 1992 Type: Article