Chitinase Gene Overexpression of Chaetomium cupreum in Pichia pastoris by Multi-site Mutations / 微生物学通报
Microbiology
;
(12)2008.
Article
in Chinese
| WPRIM
| ID: wpr-686071
ABSTRACT
By using gene SOEing PCR, Arg (CGC) in the expression fragment of chitinase gene (chi58) from Chaetomium cupreum was mutated synonymously to Arg (AGA), which is a bias code of Pichia pas-toris yeast. The expression plasmid pPIC9K-chi58A was constructed and transformed into GS115 strain through electroporation. The chitinase activity reached 101.71 U/mL?3.33 U/mL after induced with 120 h, which was the 3 fold of the original strain (31.83 U/mL?4.85 U/mL). Additionally, recombinant yeast showed good genetic stability after 10 cycle. SDS-PAGE analysis suggested that the weight of protein was 58 kD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Microbiology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS