MAR-FISH Technique and Its Application in Study of Environmental Microbial Community and Function / 微生物学通报
Microbiology
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-686161
ABSTRACT
The major goal of microbial ecology is to study the structure and function of complex micro-bial communities. New molecular biological techniques have been successfully applied to analyze mi-crobial community structure. However they do not provide information on the physiologic properties of the detected microorganisms. A new tool for structure-function analyses in microbial ecology, micro-autoradiography combined with fluorescence in situ hybridization (MAR-FISH) can be used to simul-taneously examine the phylogenetic identity and the specific activity of microorganisms within a com-plex microbial community at a single-cell level. This article reviews the principle, experimental steps of MAR-FISH technique. The application of this technique in study of the environmental microbial com-munity and function is also summarized.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Microbiology
Year:
1992
Type:
Article
Similar
MEDLINE
...
LILACS
LIS