Your browser doesn't support javascript.
loading
The Interaction Between Human TRIM5? Chimera and HIV-1gag Protein in Vitro / 中国生物工程杂志
China Biotechnology ; (12)2006.
Article in Chinese | WPRIM | ID: wpr-686256
ABSTRACT

Objective:

To express and purify the TRIM5? chimaera[TRIM5? H(R328-332)] protein and to explore the interaction between the TRIM5? H(R328-332)and HIV-1gag.

Methods:

The plasmid pET28aTRIM5? H(R328-332) was transformed to E.coli BL21 (DE3) strain ,and the expression of TRIM5? H(R328-332) protein was induced by IPTG,purified with Ni2+ chromatography.The expression and purification of TRIM5? H(R328-332) were analyzed by SDS-PAGE and Western blot,and the interaction between TRIM5? H(R328-332) and HIV-1gag was detected by co-immunoprecipitation,His pull-down and ELISA.

Results:

The recombinant plasmid pET28aTRIM5? H(R328-332) was successfully expressed in E.coli. The results showed that the purified full length TRIM5? H(R328-332) interacted with HIV-1gag protein.

Conclusion:

The human TRIM5? chimaera was expressed successfully in vitro,and the study demonstrates that the human TRIM5? chimaera interacts with HIV-1 gag in vitro.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Biotechnology Year: 2006 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Biotechnology Year: 2006 Type: Article