Purification and Characterization of Laccase from Monodictys asperospera (Cooke & Massee) Ellis / 微生物学通报
Microbiology
;
(12)2008.
Article
in Chinese
| WPRIM
| ID: wpr-686414
ABSTRACT
A new wood-degrading fungus Monodictys asperospera(Cooke & Massee) Ellis with a high level of laccase production was chosen to study.This laccase was purified by ammonium sulfate precipitation,DEAE-cellulose and sephacryl S-300.Purification of about 8.1 fold was achieved with an overall yield of 5.7%.Its molecular weight was estimated to be about 77 kD.The optimum temperature and pH of the lac-case activity were 55?C and 6.0,respectively.Kinetic studies of the laccase showed that the Km and the Vmax for using syringaldazine as substrate was 0.163 mmol/L and 0.194 mmol/(L.min),respectively.The carbo-hydrate content was 18.14%.In addition,it was found that laccase activity was significantly inhibited by Cu2+.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Microbiology
Year:
2008
Type:
Article
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