Research on Alliinase Immobilization and Its Properties / 中国生物工程杂志
China Biotechnology
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-686432
ABSTRACT
Alliinase is immobilized on pre-activated chitosan microspheres by glutaraldehyde.The optimum immobilization conditions are as follows glutaraldehyde concentration is 4%,alliinase concentration 20.2U,immobilization time 2h.For immobilized alliinase,the highest activity was allowed at pH 7.0 and temperature 35℃,and the Michaelis constant(Km)was disclosed to be 7.9mmol/L by Lineweaver-Burk plot,and after 10 times reuses the immobilized alliinase lost no more than 10% activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Biotechnology
Year:
2006
Type:
Article
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