ANTIBACTERIAL ACTIVITY OF TRADITIONAL MEDICINE “TIISHAL” AND ITS FIVE COMPONENT HERBS / Шинэ санаа Шинэ нээлт

ABSTRACT

@#BACKGROUND. Traditional medicine is the oldest form of health care in the world and is used in the prevention and treatment of physical and mental illnesses3. Traditional medicine is also variously known as complementary and alternative, or ethnic medicine, and it still plays a key role in many countries today11. Plant produces a wide variety of secondary metabolites which are used either directly as precursors or as lead compounds in the pharmaceutical industry. It is expected that plant extracts showing target sites other than those used by antibiotics will be active against drug resistant microbial pathogens7. Antibacterial activities of various extracts, including methanol, ethanol, butanol and ethyl acetate crude extracts from traditional Tiishal medicine and its medicinal plants ingredients were carried out. Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus luteus, Salmonella enterica. For this purpose extract of drug Tiishal were prepared and tested by “Disc Diffusion Method”. As a result of this study it was found that the extract of Tiishal generally revealed antimicrobial activity against both gram positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus luteus) and gram-negative bacteria (Salmonella enterica). The to study found that antibacterial activity of the ethanol extracts of each 6 samples showed little inhibition on Sal. enterica. METHODS. Traditional medicine Tiishal was produced from the Manba Datsan clinic and training center for traditional Mongolian medicine. Tiishal medicine was prepared by the standard MNS 55852006, № 0333151207 Tiishal medicine instructional method. The main medicinal herbs of Tiishal include Juniperus pseudosabinaFisch., Gentiana barbata Froel., Cynomorium songaricum Rupr., Sophora alopecuroides L., and Tricholoma mongolicum Imai (11111) ratio. The antimicrobial activity of the ethanol, methanol, butanol and ethyl acetate extracts was carried by disc diffusion method. RESULTS. A total 4 strains were used for the antibacterial activity test. The extracts of methanol, and ethanol of J. pseudosabina revealed the highest antibacterial activity against Bac. subtilis, Ps. aeruginosa, S. aureus, and S. enteric with the diameters of inhibition zones between 6.0 - 10.0 mm. СONCLUSION. “Tiishal”, ethanol and methanol extracts of 5 different plants showed relatively low inhibition of bacterial growth.