Effects of Cryopreservation on Morphological Characteristics of Washed Spermatozoa from Normozoospermia

ABSTRACT

Introduction: Successful cryopreservation of spermatozoa must ensure normal newborns after the preservation time. This method frequently can potentially contain cross-infected risks during the cryopreservation process in the liquid nitrogen environment (such as HCV, HIV). A number of researchers reveal that these risks can be eliminated by washing spermatozoa before cryopreservation. However, the problem is whether cryopreservation of washed spermatozoa still retains its morphology and function or not? \r\n', u'Objectives: To evaluate the change of sperm morphology characteristics after which washed sperm cryopreserved from normozoospermia. \r\n', u'Subjects and method: 30 normal semen samples; each sample was divided into two aliquots of washed and unwashed spermatozoa. All samples were cryopreserved in stages of 1, 2 and 30 days. We compared the percentage of spermatozoa with normal morphology before and after which was washed, pre - cryopreservation and post - cryopreservation between the groups. \r\n', u'Results: The percentage 0 spermatozoon with normal morphology after washing was more significantly increased than prior to washing. Post - cryopreservation, this percentage was reduced time by time but acceptable. There is no significant difference between the two ways of preparation before cryopreservation. The percentage of spermatozoa with abnormal head and neck increased significantly after cryopreservation. \r\n', u'Conclusion: The percentage of spermatozoa with normal morphology post - cryopreservation was reduced in both washed sperm and unwashed sperm samples. This percentage was reduced time by time, but there is no difference between the two groups studied. \r\n', u'\r\n', u'