Effect of IKK2 Inhibitor LY2409881 on Diffuse Large B-Cell Lymphoma Cell Apoptosis and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1086-1092, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-689524
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects and its potential mechanism of IKK2 inhibitor LY2409881 on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.</p><p><b>METHODS</b>CCK8 assay was used to detect the effect of LY2409881 on proliferation of DLBCL cell lines; Flow cytometry was used to analyze cell cycle; Western blot was used to analyze apoptosis and its potential mechanism.</p><p><b>RESULTS</b>LY2409881 inhibited the proliferation of multiple DLBCL cell lines obviously, and caused G cell arrest. Furtherly, LY2409881 inhibited the expression of c-FLIP, induced the activation of DR4 and caspase 8. Meanwhile, LY2409881 induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1 and BCL-2.</p><p><b>CONCLUSION</b>LY2409881 inhibits the proliferation of DLBCL cell lines, causes G cell arrest and induces apoptosis via the endogenous and exogenous apoptotic pathways.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pyrimidines
/
Thiophenes
/
Lymphoma, Large B-Cell, Diffuse
/
Apoptosis
/
Cell Line, Tumor
/
Cell Proliferation
/
I-kappa B Kinase
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2018
Type:
Article
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