The Effects of Interleukin-17 on Production of Vascular Endothelial Growth Factor in Cultured Rheumatoid Arthritis Synoviocytes / 대한류마티스학회지
The Journal of the Korean Rheumatism Association
;
: 160-168, 2001.
Article
in Korean
| WPRIM
| ID: wpr-68993
ABSTRACT
OBJECTIVE:
To investigate the the effects of interleukin-17 (IL-17)on the production of vascular endothelial growth factor (VEGF)from cultured rheumatoid arthritis synoviocytes.METHODS:
Fibroblast-like synovial cells(FLS)were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of IL-17, IL-17 with or without transforming growth factor-beta(TGF-beta),tumor necrosis factor-alpha(TNF-alpha)and interleukin-1 beta(IL-1 beta).VEGF levels were determined in the culture supernatants by sandwitch ELISA.RESULTS:
Stimulation of FLS by serial concentration of IL-17,TGF-beta,TNF-alpha,IL-1 beta increased the production of VEGF by 2.1-2.7,2.2-3.0,2.0-2.9,2.3-3.1 fold over the constitutive levels of unstimulated FLS.Stimulation of FLS by IL- 17 with TGF-beta or TNF-alpha or IL-1 beta also increased the production of VEGF accord-ing to culture periods by 1.6-1.8,1.1-1.9,1.5-1.7 fold over the levels stimulated with TGF-beta or TNF-alpha or IL-1 beta,respectively.This results indicated that IL-17 increased the effect of TGF-beta,TNF-alpha,IL-1 beta on FLS,leading synergistic enhancement of VEGF production.CONCLUSION:
IL-17 may be involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arthritis, Rheumatoid
/
Synovitis
/
Enzyme-Linked Immunosorbent Assay
/
Transforming Growth Factor beta
/
Interleukin-1
/
Tumor Necrosis Factor-alpha
/
Interleukin-17
/
Vascular Endothelial Growth Factor A
/
Interleukin-1beta
/
Necrosis
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Rheumatism Association
Year:
2001
Type:
Article
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