Two-plasmid scarless genetic modification in Escherichia coli hfq and rne-710 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 602-612, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-690143
ABSTRACT
Gene modification is an important technique to understand gene function. We firstly constructed ΔhfqSpe and Δrne-710Spe mutant strains of Escherichia coli MG1655. The fragment lacking of hfq and rne-710 was ligated to the auxiliary plasmid and separately replace the spectinomycin box by homologous recombinase system to obtain the Δhfq and Δrne-710 mutant strains. The combination of two-plasmid scarless genetic modification and fusion PCR led to a new way for the long DNA fragment gene deletions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2018
Type:
Article
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