Identification of Dioscorea nipponica and its sects based on psbA-trnH barcode / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 938-944, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-690536
ABSTRACT
A rapid and accurate method was established by psbA-trnH sequence to distinguish Dioscorea nipponica from other species belonging to the same genus.In this study, we collected 144 samples of D. nipponica from 17 different producing areas in China, the psbA-trnH sequenced of 23 nucleotide sequences were downloaded from GenBank, the sequences from Dioscorea genus. DNAMAN 8.0 software was used to show splicing, MEGA 6.0 software was applied for sequence analysis and comparison. The results showed that the genetic relationship between D. nipponica and D. subcalva was the closest, and the genetic relationship with D. zingiberensis was the furthest. It is indicated that the psbA-trnH sequence as a DNA barcode can effectively distinguish between D. nipponica and other plants in the genus of Dioscorea.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2018
Type:
Article
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