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Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 499-506, 2018.
Article in English | WPRIM | ID: wpr-690628
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling.</p><p><b>METHODS</b>HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively.</p><p><b>RESULTS</b>The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels.</p><p><b>CONCLUSION</b>YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Endopeptidases / Physiology / Thiolester Hydrolases / Signal Transduction / Keratinocytes / Cell Movement / Cells, Cultured / Cell Proliferation / Smad Proteins / Transforming Growth Factor beta3 Limits: Humans Language: English Journal: Biomedical and Environmental Sciences Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Endopeptidases / Physiology / Thiolester Hydrolases / Signal Transduction / Keratinocytes / Cell Movement / Cells, Cultured / Cell Proliferation / Smad Proteins / Transforming Growth Factor beta3 Limits: Humans Language: English Journal: Biomedical and Environmental Sciences Year: 2018 Type: Article