Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 499-506, 2018.
Article
in English
| WPRIM
| ID: wpr-690628
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling.</p><p><b>METHODS</b>HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively.</p><p><b>RESULTS</b>The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels.</p><p><b>CONCLUSION</b>YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Endopeptidases
/
Physiology
/
Thiolester Hydrolases
/
Signal Transduction
/
Keratinocytes
/
Cell Movement
/
Cells, Cultured
/
Cell Proliferation
/
Smad Proteins
/
Transforming Growth Factor beta3
Limits:
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2018
Type:
Article
Similar
MEDLINE
...
LILACS
LIS