Inhibition of Erythroid Differentiation of Human Leukemia K562 Cells by N-acetylcysteine and Ascorbic Acid through Downregulation of ROS / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 247-251, 2018.
Article
in English
| WPRIM
| ID: wpr-690663
ABSTRACT
This study investigated the effects of N-acetylcysteine (NAC) and ascorbic acid (AA) on hemin-induced K562 cell erythroid differentiation and the role of reactive oxygen species (ROS) in this process. Hemin increased ROS levels in a concentration-dependent manner, whereas NAC and AA had opposite effects. Both NAC and AA eliminated transient increased ROS levels after hemin treatment, inhibited hemin-induced hemoglobin synthesis, and decreased mRNA expression levels of β-globin, γ-globin, and GATA-1 genes significantly. Pretreatment with 5,000 μmol/L AA for 2 h resulted in a considerably lower inhibition ratio of hemoglobin synthesis than that when pretreated for 24 h, whereas the ROS levels were the lowest when treated with 5,000 μmol/L AA for 2 h. These results show that NAC and AA might inhibit hemin-induced K562 cell erythroid differentiation by downregulating ROS levels.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Acetylcysteine
/
Ascorbic Acid
/
Down-Regulation
/
Cell Differentiation
/
Reactive Oxygen Species
/
K562 Cells
/
Erythroid Cells
/
Hemin
/
Metabolism
Limits:
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2018
Type:
Article
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