Effect of MiR-99a-5p on the Differential Ability of Human Bone Marrow Mesenchymal Stem Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 563-568, 2018.
Article
in Zh
| WPRIM
| ID: wpr-690949
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of microRNA-99a-5p (miR-99a-5p) on differentiation ability of human bone marrow mesenchymal stem cells (BM-MSC).</p><p><b>METHODS</b>BM-MSC was cultured and then transfected with miR-99a-5p mimics or inhibitors. The transfection efficiency was detected by real-time quantitative PCR. The effects of miR-99a-5p on the adipogenic and osteogenic differentiation ability of BM-MSC were detected by differentiation experiment.</p><p><b>RESULTS</b>As compared with the negative control group, the expression of miR-99a-5p was significantly up-regulated after transfection with miR-99a-5p mimics(P<0.001), the expression of miR-99a-5p was down-regulated after transfection with miR-99a-5p inhibitor (P<0.001). In osteogenic differentiation experiments, the miR-99a-5p overexpression could promote the osteogenic differentiation, while the downregulation of miR-99a-5p expression inhibited the osteogenic differentiation. The same results were obtained by semi-quantitative detection through spectrophotometry. In the adipogenic differentiation test, transfection of miR-99a-5p mimics or inhibitors had no significant effect on the adipogenic differentiation of BM-MSC.</p><p><b>CONCLUSION</b>Overexpression of miR-99a-5p can promote the osteogenic differentiation of BM-MSC, but no significant effects are observed in the adipogenic differentiation.</p>
Full text:
1
Index:
WPRIM
Main subject:
Osteogenesis
/
Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
MicroRNAs
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2018
Type:
Article