Application of Epstein-barr virus antibody detection in diagnosis of nasopharyngeal carcinoma and infectious mononucleosis / 安徽医科大学学报

ABSTRACT

Objective To explore the value of Epstein-barr virus(EBV) antibody detection in diagnosis of nasopharyngeal carcinoma (NPC ) and infectious mononucleosis (IM). Methods ELISA and CLIA were used to evaluate the EBV 5 antibody in 535 hospitalized and outpatients. The results of the combined detection of EBV virus were analyzed by clinical pathological diagnosis. Results There was no statistical difference between the results of antibody tests in NPC and IM. The positive pattern of EB antibody spectrum in NPC was the highest in EBV-IgA, EBNA-IgG, EBVCA-IgG, EA-IgG mode, accounting for 84. 48％ of the positive rate, and IM was relatively more in EBVCA-IgG, EBVCA-IgM, EA-IgG mode, accounting for 54. 16％ of the total positive rate. The sensitivity of EB-NA-IgG and EBVCA-IgG in diagnosis of NPC was 100. 0％, and the specificity was 11. 3％ and 8. 5％ respectively. The specificity of EBVCA-IgM to NPC was as high as 95. 8％, while the sensitivity was only 1. 7％. In the IM, the sensitivity, specificity, accuracy and negative predictive value of the EBVCA-IgM single test were more than 90. 0％. The specificity and accuracy of EA-IgG reached 94. 5％ and 93. 1％, and the sensitivity was 70. 8％. Compared with the positive rate, the EBV-IgA and EA-IgG in NPC group were statistically significant(P <0. 05). EBVCA-IgM and EA-IgG was significantly higher than other children's disease group in IM group, the difference was statistically significant (P <0.05). Conclusion The positive rates of EBNA-IgG and EBVCA-IgG in adult diseases were higher than that in children, and the positive rate of EBVCA-IgM was significantly higher in children with IM than that in adult diseases and other children's diseases.