Study on the mechanism of miR-17-5p in atherosclerotic mice / 安徽医科大学学报

ABSTRACT

Objective To investigate the effect of miR-17-5p on the inflammatory response and oxidative stress in the development of atherosclerosis. Methods ApoE-/ - mice were fed with high fat diet for 12 weeks to con-struct atherosclerosis (AS) model, and then miR-17-5p antagonist was injected to the caudal vein of mice subse-quently. HE staining was used to observe the pathological changes of the aorta. The apoptosis of aorta cells was de-tected by TUNEL staining. Western blot was used to detect the expression of apoptosis-related proteins and the ex-pression of inflammatory cytokines weas detected by ELISA. Oxidative stress were assessed by malondialdehyde ( MDA) levels and the activity of superoxide dismutase ( SOD) and myeloperoxidase ( MPO) . Results Inhibition of miR-17-5p alleviated the aortic lesion and apoptosis in AS mice, reduced the expression of Bax, cleaved-caspase-3 and cleaved-PARP and up-regulated the expression of Bcl-2. After inhibition of miR-17-5p, the levels of TNF-α and IL-6 in AS mice decreased, and the level of IL-10 increased. Inhibition of miR-17-5p also resulted in decrease in MDA and MPO activity, but increase in SOD activity. Conclusion Inhibition of miR-17-5p can atten-uate atherosclerotic lesions by reducing the inflammatory response and oxidative stress in the aorta of atherosclerotic mice. MiR-17-5p may be a potential target for the treatment of atherosclerosis.