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Effects of Exendin-4 on expressions of lipid metabolism related genes in HepG2 cells with insulin resistance / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition) ; (6): 36-40,后插4, 2018.
Article in Chinese | WPRIM | ID: wpr-691520
ABSTRACT

Objective:

To investigate the effects of Exendin-4 (Ex-4) on the expressions of lipid metabolism related genes in the human liver cancer HepG2 cells with insulin resistance (IR),and to elucidate the effect of Ex-4in improvement of IR.

Methods:

The HepG2 cells in logarithmic growth phase were induced into IR model with high concentration of insulin,then divided into control group (HepG2 cells),IR group (HepG2 cells were treated with insulin,HepG2-IR cells),and Ex-4 group (HepG2-IR cells were treated with Ex-4).Glucose oxidase (GOD-POD)kit was used to detect the consumption of glucose.The cell morphology and intracellular lipid drip formation were observed by Oil red O staining.The triglyceride (TG) level in cells was detected by kit;qRT-PCR was used to detect the mRNA expression levels of acetyl-CoA carboxylase (ACC),fatty acid synthase (FAS),sterol regulatory element-binding protein-1c (SREBP-1c) and apolipoprotein B100 (apoB100).

Results:

Compared with control group (HepG2 cells),the glucose consumption in the HepG2-IR cells in IR group was significantly decreased (P<0.01).Compared with IR group,the glucose consumption in the HepG2-IR cells in Ex-4 group was increased (P<0.05).The Oil O red staining results showed that compared with control group,the fat percentage in the HepG2-IR cells in IR group was increased (P<0.05);compared with IR group,the fat percentage in Ex-4 group was decreased (P<0.05).Compared with control group,the level of TG in the cells in IR group was significantly increased (P<0.01);compared with IR group,the level of TG in the cells in Ex-4 group was significantly decreased (P<0.05).The qT-PCR results showed that compared with control group,the expression levels of ACC FAS and SREBP-1cmRNA in the cells in IR group were increased (P<0.01),and the expression level of apoB100 mRNA was decreased (P<0.05);compared with IR group,the expression levels of ACC,FAS and SREBP-1c mRNA in the cells in Ex-4 group were decreased (P<0.05),and the expression level of apoB100 mRNA was increased (P<0.01).

Conclusion:

Ex-4 can regulate the expressions of lipid metabolism related genes in the HepG2 cells and improve IR.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Jilin University(Medicine Edition) Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Jilin University(Medicine Edition) Year: 2018 Type: Article