Effects of Xiaoaiping combined with X-rays radiation on proliferation and apoptosis of human liver cancer HepG2 cells / 吉林大学学报(医学版)

ABSTRACT

Objective:To detect the changes of the proliferation,cell cycle and apotopsis of liver cancer HepG2cells after treated with Xiaoaiping (XAP) combined with X-rays radiation,and to clarify their anti-tumor mechanisms.Methods:The human liver cancer HepG2 cells at logarithmic phase were selected,and divided into control,XAP,2 Gy radiation and XAP + 2 Gy radiation groups.The HepG2 cells were treated with 75 mg · L-1final concentration of XAP,and they were irradiated with 2 Gy X-rays 12 h later.The cell proliferation activity was detected with CCK8 kits,the cell cycle and apoptotic rates were measured by FCM with PI single staining and Annexin Ⅴ-FITC double staining,and the caspase-3 protein expression was measured by Western blotting method.Results:After the cells were treated with XAP,the proliferation activities in XAP,2 Gy radiation and XAP+2 Gy radiation groups were significantly decreased compared with control group at 12,24 and 48 h (P＜0.05 or P＜0.01);the percentages of cells at S and G2/M phases in XAP,2 Gy radiation and XAP + 2 Gy radiatin groups were significantly increased compared with control group (P＜ 0.05 or P＜ 0.01);the apoptotic rates were significantly increased compared with control group (P＜0.05 or P＜0.01),especially in XAP + 2 Gy radiation group.The Western blotting results showed that caspase-3 was cleaved into 17 000 and 19 000 fragments,and its expression was also increased compared with control group.Conclusion:XAP combined with X-rays radiation could inhibit the proliferation,and induce the G2/M arrest and the apoptosis of liver cancer H epG2 cells;the combination of them shows a synergistic anti-tumor effect.