Influence of indoor decoration volatile pollutants in reproductive function and sperm apoptosis of male mice / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6): 281-285, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-691564
ABSTRACT
Objective:
To simulate the pollution environment of indoor decoration volatile pollutants,and to explore the influence of three main indoor decoration volatile pollutants formaldehyde,xylene and ammonia in the male mouse reproductive function.Methods:
A total of 20 male ICR mice were randomly divided into experimental group(exposed to 60 mg·m-3formaldehyde,50 mg·m-3xylene and 40 mg·m-3ammonia mixed in the static type canister)and control group(put into the air-filled static type canister)(n=10).Each mouse was weighed at set times every day,exposed for 35 d continuously.The behavior changes of the mice were also observed everyday. The epididymal cauda sperms were collected to detect the sperm density and vitality.The sperm malformation rate was detected by Diff-Quick dyeing method.The apoptotic rate of epididymal cauda sperms was detected by flow cytometry(FCM).Then Western blotting method was used to determine the expression levels of Caspase-9 and Cleaved Caspase-3 proteins in testis tissue of the mice.Results:
Four weeks after the exposure to pollutants,the body weight of the mice in experimental group was significantly lower than that in control group(P<0.01).The concentration and vitality of sperm malformation rate of the mice in experimental group were significantly lower than those in control group(P<0.01)35 d after exposure.Compared with control group,the apoptotic rate of sperm of the mice in experimental group was significantly increased(P< 0.01).However,the expression levels of Caspase-9 and Cleaved Caspase-3 in the testis tissue of the mice in experimental group and control group had no significant differences(P>0.05).Conclusion:
The main pollutants in indoor decoration can significantly influence the quality of spermatozoa in the epididymis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Jilin University(Medicine Edition)
Year:
2018
Type:
Article
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