Modified primary culture method of mouse olfactory sensory neurons and its identification / 重庆医学

ABSTRACT

Objective To establish a convenient method for primary culture of olfactory sensory neurons(OSNs).Methods The C57BL/6 mouse embryos olfactory mucosa in the mouse with gestation of 15? 20 d was collected.Then the OSNs primary culture was conducted by adopting the routine and modified separation procedure.The cellular morphology was observed on 1,3,5,7,9 d after plating by using inverted microscope.The OSNs specific β-tubulin Ⅲ antigen and olfactory marker protein(OMP) in the cultured cells were identified by using the routine immunofluorescence method.Results According to modified experimental process,partial survival cells demonstrated the morphology of typical bipolar neuron,the cells on 5 d were verified as OSNs by β-tubulin Ⅲ and OMP immunofluorescence labeling.By adopting the common process,the fibrocytes began to grow on day 3 and gradually covered the culture dish.Conclusion This research successfully establishes a relatively simple and convenient method for primary culture of OSNs.