Analysis on microbial community diversity in gallbladder stone and mucosa based on Illumina Miseq platform / 重庆医学

ABSTRACT

Objective To investigate the microbial community diversity in stone and mucosa from the patients with gallbladder stone.Methods The gall bladder mucosal samples in 60 cases(case group) of gallbladder stone treated by cholecystectomy amd 11 cases (control group)of liver right anterior lobe hemangioma in this hospital from January to December 2014 were selected for conducting the bacterial culture.The stone and mucosa in the patients with negative culture results were performed the Illumina Miseq sequencing analysis.Results A total of 71 cases were included in the study.The microbial community DNA in the control group samples was not detected out.The bacterial DNA detection rate of samples in the case group was 94.2％;in regardless of the type of sample,the most dominant was Phylum Proteobacteria.The composition proportion of stone and mucosal microbial community in the patients with same type of stone was relatively consistent,whereas different types of composition had large difference.Conclusion Using the Illumina Miseq sequencing analysis can effectively evaluate the gallbladder microbial community diversity in the patients with gallbladder stone.