Influence of endothelin A receptor antagonist pretreatment on renal function after cardiopulmonary bypass in beagles / 重庆医学

ABSTRACT

Objective To observe the influence of endothelin A receptor antagonist pretreatment on renal function after cardiopulmonary bypass (CPB) in beagles.Methods A total of 18 male beagles were selected and allocated to 3 groups (n =6) by adopting the random number table method:sham operation group (Sham group),CPB group and endothelin A receptor antagonist (ETA) group.Sitaxsentan 0.7 mg/kg in the ETA group was infused by continuous pumping for 30 min at 1 h prior to CPB.The body temperature,mean arterial pressure (MAP) and arterial gas were collected.The concentrations of serum creatinine (Scr) and blood urea nitrogen (BUN) were detected.The renal tubular necrosis score was evaluated,and the expression levels of phosphorylated Akt (p-Akt),phosphorylated eNOS (p-eNOS) were also detected.Results Serum SCr and BUN levels at 2 h after CPB in the CPB and ETA group were significantly higher than those before operation (P＜0.05),and the ETA group was obviously lower than the CPB group (P＜0.05);the renal tubular necrosis score in the ETA group was obviously lower than that in the CPB group (P＜0.05).Expressions of p-Akt,p-eNOS in the ETA group were significantly higher than those in the CPB group(P＜0.05).Conclusion CPB might contribute to acute kidney injury,the endothelin A receptor antagonist pretreatment might alleviate acute kidney injury after CPB.