Study on the relationship between HPA and NRF2 and the occurrence and development of laryngeal squamous cell carcinoma / 重庆医学

ABSTRACT

Objective To investigate the expression of heparanase (HPA) and NF-E2 associated factor (NRF2) in laryngeal squamous cell carcinoma.Methods We collected 81 cases of laryngeal squamous cell carcinoma in our hospital from February 2015 to February 2017 as group A,and then selected 77 cases of polyp of vocal cord pathological specimens as group B.The expression of HPA was detected by immunohistochemistry,and the expression of NRF2 was detected by western blot.Results The positive expression rate of HPA and the expression of NRF in the group A and B were 81.48％,19.48％ and 0.844±0.113,0.202±0.094,the difference was statistically significant (P＜ 0.05).The positive expression rates of HPA in patients with lymph node metastasis and TNM stage Ⅲ to Ⅳ were 93.02％ and 94.87％,which were significantly higher than those without lymph node metastasis and stage Ⅰ to Ⅱ (P＜0.05).The positive expression rates of HPA in patients with low and middle differentiation were 93.75％ and 100.00％,which were significantly higher than those with high differentiation (P＜0.05).The expression of NRF2 in patients with lymph node metastasis and TNM stage Ⅲ to Ⅳ were 0.901± 0.122 and 0.885 ± 0.105,which significantly higher than those without lymph node metastasis and stage Ⅰ to Ⅱ (P＜0.05).The expression of NRF2 in patients with moderate and low differentiation were 0.854± 0.101 and 0.878 ± 0.099,which were significantly higher than patients with stage Ⅰ to Ⅱ (P＜0.05).Conclusion The expression of HPA and NRF2 in laryngeal squamous cell carcinoma are significantly increased,which are related to lymph node metastasis,TNM stage and pathological grade.