Construction and identification of pseudomonas aeruginosa PA0745 gene mutant strains / 重庆医学

ABSTRACT

Objective Constructed Pseudomonas aeruginosa PA14 PA0745 mutant and verified its pathogenicity.Methods Constructed Pseudomonas aeruginosa PA0745 gene deletion and point mutant by homologous recombination.Pseudomonas aeruginosa mutant strains E126A,E146A and △PA0745 were screened by plasmid resistance and auxotrophy.Analysed mutant PA0745,PA14 growth characteristics and pathogenic changes.Results PA14 PA0745 mutant strains E126A,E146A and △PA0745 were successfully constructed.The results of growth curve showed that PA14 PA0745 mutation did not affect PA14 normal growth.The results of cell infection showed that the infection ability of E126A,E146A and △PA0745 decreased by 29.51％,60.66％ and 79.34％ respectively compared with PA14.Conclusion PA14 PA0745 mutant was successfully constructed in this study,and the function of PA0745 was confirmed,which had some guiding significance for the development of antibacterial drugs.