Study on relationship between interaction of estrogen, progesterone and relaxin with stress urinary incontinence during pregnancy / 重庆医学

ABSTRACT

Objective To study the relationship between the expression levels of estrogen,progesterone,relaxin(RLX) and expression levels of vaginal wall hormone receptors in late pregnancy with stress urinary incontinence(SUI) during pregnancy.Methods Ninety-three healthy pregnant women were selected from July 2014 to November 2015,including 47 cases in the SUI group and 46 cases in the non-SUI group.The blood sample was collected at 37-40 weeks gestation.The levels of serum E2,E3,P and RLX were respectively detected by using the chemiluminescence immunoassay and enzyme linked immunosorbent assay(ELISA).The full thickness tissues of posterior vaginal wall were obtained at delivery in 42 cases of the SUI group and 44 cases of the non-SUI group,and the expressions of ER,PR and LGR7 in pelvic floor tissues were detected by immunohistochemical staining.Results There was no statistically significant difference in serum E2,E3 and P levels between the SUI group and the non-SUI group(P＞0.05);the serum RLX level of the SUI group was significantly higher than that of the non-SUI group(P＜0.05).The positive expression intensity of ER in pelvic floor tissue of the SUI group was lower than that of the non-SUI group(P＜0.05),the positive expression intensity of PR and LGR7 in the SUI group was higher than that in the non-SUI group(P＜0.05).The serum RLX level and expression of LGR7 in pelvic floor tissues of pregnant women had no correlation with E2,E3 and P(P＞0.05).The LGR7 level in pelvic floor tissues had no correlation with ER,and was positively correlated with PR.Conclusion In late pregnancy,the biological activity of estrogen,progesterone and RLX on pelvic floor support tissues is mediated by their receptors expression levels,the progesterone receptor may have a positive regulation effect on LGR7,which is closely related to the occurrence of SUI in pregnancy.