Analysis on sensitivity of β-lactam/β-lactamase inhibitors to bifidobacteria and lactobacilli / 重庆医学

ABSTRACT

Objective To analyze the susceptibility situation of bifidobacteria and lactobacilli from child intestinal tract on β-lactam/β-lactamase inhibitor complex.Methods According to the method of the Bergey Bacterial Identification Manual,45 strains of bifidobacteria and 39 strains of lactobacilli were isolated from the stool specimens of 36 healthy children.The susceptibility tests of bifidobacteria and lactobacilli to ampicillin,amoxicillin/clavulanate,ampicillin/sulbactam,piperacillin,piperacillin/tazobactam,ticarcillin,ticarcillin/clavulanate,cefoperazone and cefoperazone/sulbactam were performed by adopting the E-test method.Then the statistical analysis was conducted.Results The sensitivity percentages of 45 strains of bifidobacteria to ampicillin,amoxicillin/clavulanate,ampicillin/sulbactam,piperacillin,piperacillin/tazobactam,ticarcillin,ticarcillin/clavulanate and cefoperazone were 77.78％,95.56％,88.89％,73.33％,91.11％,68.89％,84.44％ and 82.22％ respectively,which of 39 strains of lactobacilli to above antibiotic drugs were 74.36％,94.87％,92.31％,71.79％,92.31％,69.23％,89.74％ and79.49％,respectively.Except having no judgment due to without the CLSI drug susceptibility MIC interpretation criteria of cefoperazone/sulbactam,the sensitivity percentages of other β-lactam/β-lactamase inhibitor complex were higher than those of single β-lactam antibi otics,the sensitive number P values of bifidobacteria in ampicillin with amoxicillin/clavulanate and piperacillin with piperacillin/tazobactam were 0.01 and 0.03 respectively,which of lactobacilli in ampicillin with amoxicillin/clavulanate,ampicillin with ampicillin/sulbactam,piperacillin with piperacillin/tazobactam,ticarcillin with ticarcillin/clavulanate were 0.01,0.03,0.02 and 0.03 respectively.Conclusion β-lactam/β-lactamase inhibitors complex has stronger effect for inhibiting child intestinal tract bifidobacteria and lactobacilli than single β-lactam antibiotics.