Expression of miR-1285 in ovarian cancer tissues and its influence on proliferation and apoptosis of cancer cells / 重庆医学

ABSTRACT

Objective To explore the expression of microRNA-1285 (miR-1285) in ovarian cancer tissues,and to analyse the effects of miR-1285 expression on proliferation and apoptosis of human ovarian cancer cell lines in vitro.Methods The expressions of miR-1285 in cancer tissues and corresponding adjacent tissues from patients with ovarian cancer were detected by using fluorescence quantitative PCR.At the same time,human ovarian cancer cell lines,including SKOV3 and OVCAR3,were transfected with miR-1285 mimics in vitro,the proliferation activity of cells successfully transfected with miR-1285 was detected by MTT assay,and the apoptotic cells were detected by flow cytometry.Results The results of fluorescence quantitative PCR showed that,for patients with ovarian cancer,the expression level of miR-1285 in ovarian cancer tissues was lower than that in the corresponding adjacent tissues,the difference was statistically significant (P＜0.05).The results of experiment in vitro showed that compared with the control group,the proliferation activity of SKOV3 cells transfected with miR-1285 mimics was decreased,and the rate of apoptosis was significantly increased,there were statistically significant differences (P＜0.05);similarly,compared with the control group,the proliferation activity of OVCAR3 cells transfected with miR-1285 mimics was decreased,and the rate of apoptosis was significantly increased,there were statistically significant differences (P＜0.05).Conclusion The expression level of miR-1285 in tumor tissues of patients with ovarian cancer is decreased,which indicates that miR-1285 may be involved in the occurance and progression of ovarian cancer;the experiment in vitro shows that miR-1285 may be closely related to the proliferation and apoptosis of ovarian cancer cells.