Determination of Levofloxacin in Pork by Molecularly Imprinted Solid Phase Extraction Spectroscopy / 分析化学

ABSTRACT

A printed polymer for selective identification of levofloxacin was synthesized on the surface of silica gel with levofloxacin as template molecule. The polymer was characterized by elemental analysis and infrared spectroscopy, and the properties of the polymer were determined by dynamic adsorption and selective adsorption. The results showed that the maximum adsorption capacity of the imprinted polymer was 56.33 mg/g and the imprinting factor was 2.62. The imprinted polymer was applied to quantitative analysis of levofloxacin with molecular imprinted solid phase extraction spectroscopy (SPES). The SPES was carried out in an elaborately designed device with which the interested analyte was extracted by the solid phase extraction medium and the diffuse reflectance spectrum was measured directly on the solid medium without elution. SPES has simplified the operation process and improved the sensitivity. The regression equation of the standard curve was A=0. 0496C+0. 2412, the correlation coefficient (R2) was 0. 9924, the linear range was 0. 25-9.0 mg/L,and the detection limit was 0.24 mg/L. The recoveries of determination of levofloxacin in Pork samples were 89.1%-92.0%, and the relative standard deviations (RSDs) of the three parallel tests were 3.4%-7.9%. Compared with the traditional enrichment and separation technique, this method developed here had some advantages such as miniaturization and integration, high sensitivity and selectivity, low cost, simple operation and rapid detection.