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Detection of Arginine by AuNPs / CQDs Nanoprobes Based on Fluorescence Resonance Energy Transfer Effect / 分析化学
Chinese Journal of Analytical Chemistry ; (12): 960-968, 2018.
Article in Chinese | WPRIM | ID: wpr-692337
ABSTRACT
A novel method for rapid detection of arginine based on fluorescence resonance energy transfer effect (FRET) between carbon quantum dots ( CQDs) and gold nanoparticles ( AuNPs) was developed. Firstly, the CQDs with excellent fluorescence properties were synthesized by one-step microwave assisted method. The AuNPs/ CQDs composites were characterized and their quenching mechanism was analyzed. Then the amount of AuNPs/ CQDs, the pH value and the reaction time were optimal. Under the optimum conditions, the fluorescence system was used to detect the content of arginine, showing a good linear relationship ( R2 = 0. 993 ) between fluorescence intensity and concentration of arginine in the range of 0. 1-10. 0 μmol/ L, and the detection limit was 5. 8 nmol/ L. Finally, the content of arginine in grape juice was determined by this method with recoveries of 105. 4% -110. 8% , which indicated that the proposed FRET system had the potential for practical detection of arginine in fruit juice.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Analytical Chemistry Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Analytical Chemistry Year: 2018 Type: Article