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Microfluidic Chip for Methamphetamine Detection Based on Time-resolved Fluoroimmunoassay / 分析化学
Chinese Journal of Analytical Chemistry ; (12): 1116-1121, 2018.
Article in Chinese | WPRIM | ID: wpr-692357
ABSTRACT
A novel polydimethylsiloxane ( PDMS)-poly-L-lysine ( PLL) microfluidic chip, comprised of 24 reaction channels with 2. 5 μL volume of each reaction channel only, was proposed for quantitative analysis of methamphetamine ( MET) based on time-resolved immunoassay techniques. The chip utilized the adsorption characteristics of PDMS and PLL towards proteins to immobilize MET complete antigens ( MET-BSA) on the surface of reaction channel. Then the competition reaction could happen between MET-Ag in the sample solution and MET-BSA on the inner surfaces of the reaction channels with MET-Ab in the reagent. The surface of latex microspheres was labeled by lanthanide, which could emit red fluorescence under the exposure of ultraviolet ( UV ) . Based on the principle of competitive immunoassay, the more MET-Ag, the less the fluorescence intensity in the reaction channel. The detection results of this chip were acquired using UV-irradiation fluorescence imaging method. With this method, 24 samples could be detected and analyzed simultaneously on a chip by just taking the fluorescence image of the chip. The method allows the detection of MET antigens ranging from 100 ng/mL to 3000 ng/mL, with less sample consumption and high-throughput. This chip is suitable for the police preliminary screening work and has a good application prospects.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Analytical Chemistry Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Analytical Chemistry Year: 2018 Type: Article