Comparison and analysis of fresh blood samples in immune qualitative items results mutual recognition in Chongqing City during 2013-2016 / 国际检验医学杂志

ABSTRACT

Objective To analyze the comparability of immune qualitative items results in the clinical labo-ratory department of partial tertiary hospital and second-class hospitals of Chongqing City during 2013-2016 to lay the foundation for realizing the results mutual recognition of immune qualitative items .Methods The basic situation survey and inquiry were conducted by the questionnaire survey and spot-supervision modes .The comparison and analysis of fresh blood samples for hepatitis Bimmune markers and autoantibody items were continuously performed from 2013 to 2016 .The problems were found by the investigation guidance and fresh blood samples comparison by stages ,and then continuously improving the test quality was performed .Results The investigation and analysis found that the development in immune sub-professional group of hospital clinical laboratory department in Chongqing City was imbalance ,the resource allocation had large difference and the coverage rate of internal quality items urgently needed to be increased .In the comparison of fresh blood samples during 2013-2016 ,the items of HBsAg and HBsAb had good consistency ,followed by HBeAg . The average scores of item passing rate during 2013-2016 were 99 .23％ ,100 .00％ ,96 .40％ and 98 .72％ for HBsAg ;100 .00％ ,99 .30％ ,97 .00％ and 98 .46％ for anti-HBs ;98 .50％ ,92 .40％ ,92 .70％ and 97 .69％ for HBeAg .The results consistency of HBeAb and HBcAb was poorer than that of the former 3 items due to large difference in the reagent ,instrument and methodology .The consistency rate in the comparison the karyotype , negative and positive in the autoantibody items was 100 .00％ ,but there was few laboratories carrying out this items(<15) .Conclusion The quality of immune mutual recognition items of tertiary hospital laboratories is good ,and the test quality of partial items in the second-class hospital laboratory still needs to be further in-creased;through the immune professional investigation and analysis ,and the comparison of fresh blood sam-ples ,standardizing the internal quality control and external quality assessment can increase the comparability and accuracy of inter-hospital test results mutual recognition to lay the foundation for promoting the hierarchi-cal diagnosis and treatment .