The mechanism study of miRNA-19a impacting on biological characteristics of colon cancer LoVo cell / 国际检验医学杂志

ABSTRACT

Objective To investigate the mechanism of miRNA-19a impacting on biological characteristics of colon cancer Lovo cell.Methods miRNA-19a mimics was transfected into human colon cancer cell line LoVo cells by liposome mediated transfection.The expression of miRNA-19a gene after transfection was detected.Cell proliferation was detected by CCK8 Kit.The effect of apoptosis was detected by TUNEL method.In vitro invasiveness of cells was detected by cell invasion chamber method.Hsp90/Akt signaling pathways changes were detected by RT-PCR and Western blot.Results The expression of miRNA-19a were increased.The ability of proliferation were promoted in vitro.The cell apoptosis was reduced.The cell invasion was increased.The expression of Hsp90 and Akt gene and protein were increased.Conclusion miRNA-19a could enhance Lovo cell invasion ability by the method of raising the expression of Hsp90 and Akt signaling pathways,accelerating LoVo cell proliferation,reducing cell apoptosis.