Study on drug resistance genes of 102 strains of Pseudomonas aeruginosa based on high throughput sequencing / 国际检验医学杂志

ABSTRACT

Objective To analyze the resistance genes of multidrug-resistant Pseudomonas aeruginosa by high throughput sequencing and bioinformatics.Methods Multiple drug-resistant strains were screened from 246 strains of Pseudomonas aeruginosa.The genome and plasmid DNA of target bacteria were extracted by al-kaline lysis and sequenced by high throughput.The resistance genes were analyzed by bioinformatics.Results 102 strains of multidrug resistant strains were detected,21 strains of extensively drug resistant strains of poly-myxin B sensitive.102 strains of multidrug resistant Pseudomonas aeruginosa samples by high-throughput se-quencing generated reads 32 088 766,the total length of 98 nt,sequencing base 3.1 Gb,8 types of antibiotic re-sistance were found,containing 21 kinds of resistant genotypes,a total of 16 polymorphic loci gene had 69 nu-cleotides,including 8 genotypes of 17 loci the occurrence of non-synonymous mutations,total 24.6% SNP loci. Conclusion Analysis of the use of multi drug resistant pathogen resistant bacteria has the feasibility of high-throughput gene sequencing technology hybrid genome.