The mechanism of metformin induced human choriocarcinoma cell line JAR apoptosis / 国际检验医学杂志

ABSTRACT

Objective To investigate the possible mechanism of metformin on apoptosis of human chorio-carcinoma cell.Methods The human choriocarcinoma cell line JAR was selected and divided into control and metformin groups(final concentrations were 5,10,20,40 and 80 mmol/L).Confocal microscope was adopted to detect cell apoptosis after 48 h treatment.The mRNA and protein expression of AMPK,p-AMPK,mTOR, Caspase-3,Bcl-2 and Bax were measured by Real-time PCR and Western blot respectively.Results Compared with the control group,apoptosis rate of JAR cells in the metformin group(final concentration was 40 mmol/L)was remarkably increased.Metformin activated AMPK by phosphorylation and inhibited mTOR pro-tein expression,meanwhile mRNA and protein expression levels of Caspase-3 and Bax were significantly in-creased,but Bcl-2 mRNA and protein expression were significantly decreased.Conclusion Metformin induces the JAR cells to generate apoptosis by the AMPK/mTOR pathways and Caspase-3,Bcl-2/Bax pathways simaltaneously.