Association of CD44 gene rs13347 polymorphism with occurrence and metastasis of breast cancer / 国际检验医学杂志

ABSTRACT

Objective By exploring the relationship between the gene polymorphism of tumor related gene CD44 and the occurrence of breast cancer,the pathogenic gene or genetic predisposing gene of breast cancer was studied so as to provide theoretical basis and technical support for the screening,diagnosis and treatment of breast cancer.Methods Samples from 265 cases of breast cancer and 290 healthy female were collected. Multiple polymerase chain reaction (PCR) amplification and two generation sequencing were used to analyze the genetic polymorphism of 4 loci of CD44 rs11607862,rs13347,rs7116432 and rs8193.T he size and metasta-sis of 235 patients were compared.Results There was no significant difference in the expression of four sitesloci in breast cancer patients.However,in the analysis of cancer patients,the GG genotype of rs13347 lo-cus was lower than that of CC/CG type,and the lymph node metastasis rate was lower.Conclusion There is a certain correlation between the rs13347 loci of CD44 and the metastasis of breast cancer.The correlation be-tween the genetic polymorphisms of 3 loci of rs11607862,rs7116432 and rs8193 and the development of breast cancer needs more clinical samples and further research.