Expression of HIF-1α ,GLUT-1 and LDH-5 in gastric cancer tissues and their correlation with pathological features / 国际检验医学杂志

ABSTRACT

Objective To investigate the expression of hypoxia inducible factor 1α (HIF-1α) ,glucose trans-porter-1 (GLUT-1) and lactate dehydrogenase-5 (LDH-5) in gastric cancer tissues and their correlation with pathological features.Methods From June 2015 to June 2017 ,93 cases of gastric cancer resection were select-ed as the observation group ,and the cancer adjacent tissues were taken as the control group.Immunohisto-chemical staining was used to detect the expression of HIF-1α ,GLUT-1 and LDH-5.The gastric cancer tissues from 93 patients with gastric cancer who underwent gastrectomy in the hospital were taken as the observation group ,and the adjacent tissues were taken as the control group.The expressions of HIF-1α ,GLUT-1 and LDH-5 in two groups were determined by Immunohistochemical MaxVision Ⅲ method.The positive expres-sions of HIF-1α ,GLUT-1 and LDH-5 in two groups were compared ,and the correlation between HIF-1α , GLUT-1 and LDH-5 in gastric cancer tissues ,and the expressions of HIF-1α ,GLUT-1 and LDH-5 in different sex ,age ,clinical stage and differentiation degree were compared.Results The positive expression rates of HIF-1α ,GLUT-1 and LDH-5 in the observation group were higher than those in the control group ,and the differences were statistically significant (P＜ 0.05) ;HIF-1α ,GLUT-1 and LDH-5 were positively correlated with each other ;there were no significant differences of HIF-1α ,GLU T-1 and LDH-5 levels in different gender group and different age group (P＞0.05) ;the positive expression rates of HIF-1α ,GLU T-1 and LDH-5 at Ⅲ and Ⅳ stages were higher than those at Ⅰ and Ⅱ stages ,the positive expression rates of HIF-1α ,GLUT-1 and LDH-5 of low differentiation were higher than those of high and middle differentiation and the differences were statistically significant (P＜ 0.05).Conclusion The high expressions of HIF-1α ,GLUT-1 and LDH-5 were observed in gastric cancer tissue.HIF-1α ,GLUT-1 and LDH-5 were linearly and positively correlated with each other ,and had a significant correlation with the clinicopathological features.With the increase of clinical stage and the degree of differentiation ,the expression of HIF-1 a ,GLUT-1 and LDH-5 were higher.