The correlation study between miRNA-802 expression and inflammatory response in patients with inflammatory bowel disease / 国际检验医学杂志

ABSTRACT

Objective To investigate the correlation and significance between miRNA-802 and inflammato-ry factors in the patients with inflammatory bowel disease (IBD) ,and to understand pathologic mechanisms of IBD.Methods 80 patients with active IBD ,who were hospitalized in Department of Gastroenterology ,Taizhou People's Hospital ,from August 2015 to February 2017 ,were selected as active IBD group.Another 40 inactive IBD patients and 40 healthy persons who underwent the healthy assessment during the same period were se-lected as inactive IBD group and healthy control group.The expression level of miRNA-802 in intestinal muco-sa and peripheral blood mononuclear cells of each group was detected by fluorescence quantitative PCR (RT-qPCR).In addition ,the relative expression of miRNA-802 in the intestinal mucosa and peripheral blood mono-nuclear cells of 43 patients with Crohn's disease (CD) treated by IFX (IFX) was detected and the correlation with the serum TNF-α was analyzed.Results The expression level of miRNA-802 in the intestinal mucosa and peripheral blood mononuclear cells of active IBD patients was significantly higher than that of inactive IBD pa-tients and healthy control group ,and the difference was statistically significant (P＜0.01).After 12 weeks of IFX treatment ,the expression level of miRNA-802 in the intestinal mucosa and peripheral blood mononuclear cells of CD patients was significantly lower than that before treatment ,and the difference was statistically sig-nificant (P＜0.01).The results of correlation analysis have shown that the expression level of serum TNF-α was positively correlated with miRNA-802 expression in peripheral blood monocytes ,and the expression of miRNA-802 mRNA was significantly increased after the stimulation of TNF-α on peripheral blood monocytes cultured in vitro.Conclusion The increased expression level of miRNA-802 in IBD patients is closely correla- ted with the expression level of TNF-α ,and miRNA-802 could be a new treatment target for IBD.