Mechanism of extracts from Aspongopus chinensis Dallas on the proliferation and apoptosis of gastric cancer cell through STAT3/Survivin pathway / 国际生物医学工程杂志

ABSTRACT

Objective To investigate the regulation and mechanism of extracts from Aspongopus chinensis dallas on the proliferation and apoptosis of gastric cancer cells. Methods The human gastric cancer cell line MGC-803 was used in the experiments. The MTT assay and flow cytometry were respectively used to detect the effects of the extracts from aspongopus chinensis dallas on the proliferation and apoptosis of the cancer cells. The nude mouse model of MGC-803 cells was established. The experimental group was injected with 50μl of the extracts (12 mg/L) in the tumor area daily, and the control group was injected with the same volume of physiological saline. The size of tumor was measured regularly, and the expression levels of STAT3 and Survivin were detected by Western blotting. Results The extracts from Aspongopus chinensis dallas significantly inhibited cell proliferation (P<0.05) and promoted cell apoptosis (P<0.05). Both of the results showed a dose-dependent trend. The tumor size of the MGC-803 tumor-bearing nude mice treated with the extracts was significantly smaller than that of the control group at different time points (all P<0.05). The expression levels of STAT3 and Survivin protein in the tumor tissues of experimental groups were significantly lower than those in the control group (all P<0.05). Conclusion The extracts from Aspongopus chinensis Dallas can inhibit proliferation and promote apoptosis of gastric cancer cells. The mechanism may be achieved by reducing the expression of STAT3 and Survivin protein.