Protective effect of resveratrol on radiation-induced small intestinal injury / 国际生物医学工程杂志

ABSTRACT

Objective To investigate the protective effect of resveratrol on γ-ray radiation induced small intestinal injury in mice.Methods Sixty-four C57BL/6 male mice were divided into 7.2 Gy irradiation group,7.2 Gy irradiation+resveratrol group,15 Gy irradiation group,15 Gy irradiation+resveratrol group,17 Gy irradiation group,17 Gy irradiation +resveratrol group,resveratrol group and control group according to the method of radiation and administration.Radiation irradiation was performed with a single dose of 0.99 Gy/min.The control and single irradiation groups were given 14.4％ of ethanol solution by intragastric administration.The resveratrol group and irradiation+resveratrol groups were given a resveratrol mixed solution at a dose of 60 mg/kg.The 30-day survival rate of the mice was observed.The morphological changes of the small intestine at 96 hours after the irradiation,and the expression of the intestinal crypts at 4 hours after irradiation was examined.Results Compared with the single irradiation groups,resveratrol can significantly increase the 30-day survival rate of the mice after 7.2 Gy total body irradiation and 17 Gy abdominal irradiation (all P＜0.05).In addition,resveratrol can significantly improve the small intestinal crypt-villus structure damage in mice after 15 Gy abdominal irradiation,down-regulated the expression of p21 and Puma by 0.71 and 0.52 times,respectively (all P＜0.05),and up-regulated the expression of Nrf2,Ho1,iNos and Nqo1 by 3.03,1.62,3.94,and 4.13 times,respectively (all P＜0.05).Conclusion Resveratrol can effectively alleviate the radiation-induced small intestine injury in mice,which may be achieved by regulating anti-apoptotic and anti-oxidant gene expression.