Exploration on extraction and enrichment process of anti-inflammatoryand analgesic active-fraction(ARF)which containsmethyl salicylate glycosides in Dianbaizhu / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 884-889, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-693330
ABSTRACT
Objective The active ingredient was used as index to optimize the extraction and enrichment process of anti-in-flammatory and analgesic active-fraction(ARF)of Dianbaizhu. Methods Methyl salicylate triglycoside-B was chosen as index com-ponent to extract and enrich methyl salicylate glycosides. Extraction and elution solvents were optimized. The HPLC fingerprint was ob-tained with Thermo Hypersil Gold C18(250 mm×4.6 mm,5μm)column and a gradient elution with the mobile phase consisting of ace-tonitrile(A)-0.2%acetic acid(B)at a flow rate of 1.0 ml/min. And the detection wavelength was set at 294 nm. Results The opti-mized extraction solvent of Dianbaizhu was the 30%ethanol and the optimized elution solvent of ARF enriched by AB-8 macroporous resins was the 35%ethanol. The methodological study on similarity and RSD in ARF HPLC fingerprint of three batches of samples cor-responded to related regulations. Conclusion The extraction and enrichment process of ARF is stable and repeatable.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of International Pharmaceutical Research
Year:
2017
Type:
Article
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